Next generation sequencing (NGS), often called massively parallel or deep sequencing are related to techniques of DNA sequencing  in which millions of nucleotide sequences are deciphered simultaneously, this technology which has revolutionised genomic research.

 

In contrast, the previous Sanger sequencing technology,  a method that first emerged in 1977 and then dominated for three decades, required over 10 years to deliver the final draft. NGS minimizes the need for the fragment-cloning methods that are often used in that previous technique sequencing of genomes.

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Bioinformatics analyses are used to piece together these fragments by mapping the individual reads to the human reference genome.

 

Each of the three billion bases in the human genome is sequenced multiple times, providing high depth to deliver accurate data and an insight into unexpected DNA variation.

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WHOLE GENOME SEQUENCING (WGS)

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Whole-genome sequencing (WGS) is a comprehensive method  used to determine nearly all of the approximately 3 billion nucleotides of an individual’s complete DNA sequence, including non-coding sequence and also used to see if a patient has a genetic disorder or is at risk for a disease.  

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WHOLE EXOME SEQUENCING (WES)

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WES testing examines all the protein coding regions in the genome simultaneously, it represents 2% of the entire Genome and the disease-causing mutations are mostly located in this area.

 

WES testing examines all the protein coding regions in the genome (exons) simultaneously. It is estimated that most of the disease-causing mutations (about 85%) are located within in it.

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QUANTIQUN

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We use different sequencing depths in NGS, which can also be used in conjunction with other techniques that amplify the result and improve the quality of the report. We  also conduce studies using different algorithms to identify interactions between drugs and between genes.

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